Sp1 전사율

Sp1 transcription factor
SP1
Protein SP1 PDB 1sp1.png
사용 가능한 구조
PDBOrtholog 검색: PDBe RCSB
식별자
에일리어스SP1, entrez: 6667, Sp1 전사율
외부 IDOMIM : 189906 MGI : 98372 HomoloGene : 8276 Genecard : SP1
맞춤법
종.인간마우스
엔트레즈

6667

20683

앙상블

ENSG00000185591

ENSMUSG00000001280

유니프로트

P08047

O89090

RefSeq(mRNA)

NM_001251825
NM_003109
NM_138473

NM_013672

RefSeq(단백질)

NP_001238754
NP_003100
NP_612482

NP_038700

장소(UCSC)Chr 12: 53.38 ~53.42 MbChr 15: 102.31 ~102.34 Mb
PubMed 검색[3][4]
위키데이터
인간 보기/편집마우스 표시/편집

특이성 단백질 1*로도 알려진 전사인자 Sp1은 SP1 [5]유전자에 의해 인간에서 암호화되는 단백질이다.

기능.

이 유전자에 의해 코드된 단백질은 많은 프로모터의 GC가 풍부한 모티브에 결합하는 아연 손가락 전사 인자입니다.암호화된 단백질은 세포 분화, 세포 성장, 아포토시스, 면역 반응, DNA 손상에 대한 반응, 그리고 염색질 리모델링을 포함한 많은 세포 과정에 관여합니다.인산화, 아세틸화, O-GlcNAcylation 및 단백질 분해 처리와 같은 번역 후 변형은 활성제 또는 억제제가 [5]될 수 있는 이 단백질의 활성에 유의하게 영향을 미친다.

SV40 바이러스에서 Sp1은 게놈의 조절영역(RR)에서 GC박스에 결합한다.

구조.

SP1은 Sp/KLF 계열의 전사 인자에 속합니다.단백질은 785개의 아미노산 길이에 분자량은 81kDa이다.SP1 전사 인자는 N 말단에 프로모터 트랜스 [6]활성화에 필요한 것으로 여겨지는 2개의 글루타민이 풍부한 활성화 도메인을 포함한다.SP1은 C 말단에 세 가지 아연 핑거 단백질 모티브를 포함하고 있으며, 이를 통해 DNA에 직접 결합하고 다른 전사 조절제와의 상호작용을 가능하게 한다.그 아연핑거는 Cys2/His형이며2 컨센서스 배열 5'-(G/T)GGGG(G/A)(C/T)-3'(GC박스 요소)와 결합한다.인간 [7]게놈에는 약 1만2000개의 SP-1 결합 부위가 있다.

적용들

Sp1은 아릴 탄화수소 수용체 및/또는 에스트로겐 수용체의 증감을 연구할 때 비교되는 제어단백질로서 사용되어 왔으며, 이는 양쪽 모두에 결합하고 일반적으로 비교적 일정한 [8]수준을 유지하기 때문이다.

최근 FTMT의 추정촉진제 영역 및 양성조절제 {SP1, cAMP 반응요소결합단백질(CREB), 잉양1(YY1)] 및 음성조절제[GATA2, 포크헤드박스단백질 A1(FoxA1), CCAAT 인핸서결합단백질 B/EB]이러한 조절제의 FTMT 프로모터에 대한 DNA 결합 활성에 대한 DFP의 영향은 ChIP(Chromatin Immunopreciation) 분석을 사용하여 조사되었다.조절제 중 SP1만이 용량 의존적 방식으로 DFP 처리 후 유의미한 DNA 결합 활성을 보였다.siRNA에 의한 SP1 녹다운은 FTMT의 mRNA 수준의 DFP 유도 증가를 폐지하여 DFP의 존재 하에서 SP1 매개 FTMT 발현 조절을 나타냈다.Deferiprone에 의한 치료는 세포질 및 핵 SP1의 발현을 증가시켰으며,[9] 핵에서의 국소화가 지배적이었다.

억제제

Streptomyces Plicatus에 의해 생성되는 항종양 항생물질인 Plicamycin과 Withania somnifera 식물의 스테로이드 락톤인 [10][11]Withaferin A는 Sp1 전사인자를 억제하는 것으로 알려져 있다.

miR-375-5p 마이크로RNA는 대장암세포에서 SP1과 YAP1의 발현을 유의미하게 감소시켰다.SP1 및 YAP1 mRNA는 miR-375-5p의 [12]직접 타깃입니다.

상호 작용

Sp1 전사인자는 다음 인자와 상호작용하는 것으로 나타났습니다.

레퍼런스

  1. ^ a b c GRCh38: 앙상블 릴리즈 89: ENSG00000185591 - 앙상블, 2017년 5월
  2. ^ a b c GRCm38: 앙상블 릴리즈 89: ENSMUSG00000001280 - 앙상블, 2017년 5월
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  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
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외부 링크

이 기사에는 미국 국립 의학 도서관(미국 국립 의학 도서관)의 공공 도메인 텍스트가 포함되어 있습니다.