TDG 사용 가능한 구조물 PDB 휴먼 유니프로트 검색: PDBe RCSB PDB ID 코드 목록 1WYW , 2D07 , 2RBA , 3UFJ , 3UO7 , 3UOB , 4FNC , 4JGC , 4XEG , 4Z3A , 4Z47 , 4Z7B , 4Z7Z , 5CYS
식별자 별칭 TDG 외부 ID OMIM : 601423 MGI : 3645587 HomoloGene : 2415 GeneCard : TDG 직교체 종 인간 마우스 엔트레스 앙상블 유니프로트 RefSeq(mRNA) RefSeq(단백질) 위치(UCSC) Chr 12: 103.97 – 103.99Mb n/a PubMed 검색[2] [3] 위키다타
G/T 불일치 특정 티민 DNA 글리코실라아제 는 인간에서 TDG 유전자 에 의해 인코딩되는 효소 다.[4] [5] [6] 몇몇 박테리아 단백질은 이 단백질과 강한 염기서열 호몰로리 를 가지고 있다.[7]
함수 이 유전자가 인코딩한 단백질은 TDG/머그 DNA 글리코실라아제 계열에 속한다. 티민-DNA 글리코실라아제(TDG)는 DNA의 설탕-인산염 백본과 손상된 티민 사이의 탄소-질소 결합을 가수분해하여 G/T 불일치로부터 티민 모이에티를 제거한다. 이 효소는 낮은 활동으로 C/T와 T/T 실수의 티민도 제거한다. TDG는 또한 구아닌으로 오인하는 것에서 요람과 5-브롬ouracil을 제거할 수 있다. TDG 녹아웃 마우스 모델은 기능적 호몰로뉴 MBD4와 같은 다른 효소가 기능적 중복성을 제공할 수 있음을 암시하는 오퍼링 주파수의 증가를 보이지 않았다. 이 유전자는 12번 염색체의 p팔에 유사 유전자를 가지고 있을 수 있다.[6]
또한 2011년에도 인간의 티민 DNAglycosylase(hTDG)효율적으로 게놈 DNA.[8]에 나중에hTDG 촉매 도메인의 단지 내 이중 DNA는 크리스털 구조 5caC가 들어 있으며 실렸던 suppor5-formylcytosine(5fC)과5-carboxylcytosine(5caC), 5-methylcytosine의 주요 산화 제품을 잘라내는 보도되었다.이익 포유류 5-메틸시토신 데메틸화에서 TDG의 [9]
상호작용 티민-DNA 글리코실라아제는 다음과 상호작용 하는 것으로 나타났다.
대화형 경로 지도 각 기사에 연결하려면 아래의 유전자, 단백질, 대사물을 클릭하십시오. [§ 1]
[[파일: alt=Fluorouracil(5-FU) Activity edit ]]
참조 ^ a b c GRCh38: 앙상블 릴리스 89: ENSG00000139372 - 앙상블 , 2017년 5월^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Neddermann P, Gallinari P, Lettieri T, Schmid D, Truong O, Hsuan JJ, Wiebauer K, Jiricny J (August 1996). "Cloning and expression of human G/T mismatch-specific thymine-DNA glycosylase" . J Biol Chem . 271 (22): 12767–74. doi :10.1074/jbc.271.22.12767 PMID 8662714 . ^ Sard L, Tornielli S, Gallinari P, Minoletti F, Jiricny J, Lettieri T, Pierotti MA, Sozzi G, Radice P (December 1997). "Chromosomal localizations and molecular analysis of TDG gene-related sequences". Genomics . 44 (2): 222–6. doi :10.1006/geno.1997.4843 . PMID 9299239 . ^ a b "Entrez Gene: TDG thymine-DNA glycosylase" .^ Gallinari P, Jiricny J (October 1996). "A new class of uracil-DNA glycosylases related to human thymine-DNA glycosylase". Nature . 383 (6602): 735–8. Bibcode :1996Natur.383..735G . doi :10.1038/383735a0 . PMID 8878487 . S2CID 4235485 . ^ He YF, Li BZ, Li Z, Liu P, Wang Y, Tang Q, Ding J, Jia Y, Chen Z, Li L, Sun Y, Li X, Dai Q, Song CX, Zhang K, He C, Xu GL (September 2011). "Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA" . Science . 333 (6047): 1303–7. Bibcode :2011Sci...333.1303H . doi :10.1126/science.1210944 . PMC 3462231 PMID 21817016 . ^ Zhang L, Lu X, Lu J, Liang H, Dai Q, Xu GL, Luo C, Jiang H, He C (February 2012). "Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA" . Nature Chemical Biology . 8 (4): 328–30. doi :10.1038/nchembio.914 . PMC 3307914 PMID 22327402 . ^ Tini M, Benecke A, Um SJ, Torchia J, Evans RM, Chambon P (February 2002). "Association of CBP/p300 acetylase and thymine DNA glycosylase links DNA repair and transcription" . Mol. Cell . 9 (2): 265–77. doi :10.1016/S1097-2765(02)00453-7 PMID 11864601 . ^ Chen D, Lucey MJ, Phoenix F, Lopez-Garcia J, Hart SM, Losson R, Buluwela L, Coombes RC, Chambon P, Schär P, Ali S (October 2003). "T:G mismatch-specific thymine-DNA glycosylase potentiates transcription of estrogen-regulated genes through direct interaction with estrogen receptor alpha" . J. Biol. Chem . 278 (40): 38586–92. doi :10.1074/jbc.M304286200 PMID 12874288 . ^ Takahashi H, Hatakeyama S, Saitoh H, Nakayama KI (February 2005). "Noncovalent SUMO-1 binding activity of thymine DNA glycosylase (TDG) is required for its SUMO-1 modification and colocalization with the promyelocytic leukemia protein" . J. Biol. Chem . 280 (7): 5611–21. doi :10.1074/jbc.M408130200 PMID 15569683 . ^ a b Hardeland U, Steinacher R, Jiricny J, Schär P (March 2002). "Modification of the human thymine-DNA glycosylase by ubiquitin-like proteins facilitates enzymatic turnover" . EMBO J . 21 (6): 1456–64. doi :10.1093/emboj/21.6.1456 . PMC 125358 PMID 11889051 . ^ Minty A, Dumont X, Kaghad M, Caput D (November 2000). "Covalent modification of p73alpha by SUMO-1. Two-hybrid screening with p73 identifies novel SUMO-1-interacting proteins and a SUMO-1 interaction motif" . J. Biol. Chem . 275 (46): 36316–23. doi :10.1074/jbc.M004293200 PMID 10961991 .
추가 읽기 Lindahl T (1982). "DNA repair enzymes" . Annu. Rev. Biochem . 51 : 61–87. doi :10.1146/annurev.bi.51.070182.000425 PMID 6287922 . Hardeland U, Bentele M, Lettieri T, et al. (2001). "Thymine DNA glycosylase". Base Excision Repair . Prog. Nucleic Acid Res. Mol. Biol . Progress in Nucleic Acid Research and Molecular Biology. Vol. 68. pp. 235–53. doi :10.1016/S0079-6603(01)68103-0 . ISBN 978-0-12-540068-8 PMID 11554300 . Chevray PM, Nathans D (1992). "Protein interaction cloning in yeast: identification of mammalian proteins that react with the leucine zipper of Jun" . Proc. Natl. Acad. Sci. U.S.A . 89 (13): 5789–93. Bibcode :1992PNAS...89.5789C . doi :10.1073/pnas.89.13.5789 PMC 402103 PMID 1631061 . Neddermann P, Jiricny J (1994). "Efficient removal of uracil from G.U mispairs by the mismatch-specific thymine DNA glycosylase from HeLa cells" . Proc. Natl. Acad. Sci. U.S.A . 91 (5): 1642–6. doi :10.1073/pnas.91.5.1642 PMC 43219 PMID 8127859 . Neddermann P, Jiricny J (1993). "The purification of a mismatch-specific thymine-DNA glycosylase from HeLa cells" . J. Biol. Chem . 268 (28): 21218–24. doi :10.1016/S0021-9258(19)36913-3 PMID 8407958 . Barrett TE, Savva R, Panayotou G, et al. (1998). "Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions" . Cell . 92 (1): 117–29. doi :10.1016/S0092-8674(00)80904-6 PMID 9489705 . S2CID 9136303 . Missero C, Pirro MT, Simeone S, et al. (2001). "The DNA glycosylase T:G mismatch-specific thymine DNA glycosylase represses thyroid transcription factor-1-activated transcription" . J. Biol. Chem . 276 (36): 33569–75. doi :10.1074/jbc.M104963200 PMID 11438542 . Tini M, Benecke A, Um SJ, et al. (2002). "Association of CBP/p300 acetylase and thymine DNA glycosylase links DNA repair and transcription" . Mol. Cell . 9 (2): 265–77. doi :10.1016/S1097-2765(02)00453-7 PMID 11864601 . Hardeland U, Steinacher R, Jiricny J, Schär P (2002). "Modification of the human thymine-DNA glycosylase by ubiquitin-like proteins facilitates enzymatic turnover" . EMBO J . 21 (6): 1456–64. doi :10.1093/emboj/21.6.1456 . PMC 125358 PMID 11889051 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Abu M, Waters TR (2003). "The main role of human thymine-DNA glycosylase is removal of thymine produced by deamination of 5-methylcytosine and not removal of ethenocytosine" . J. Biol. Chem . 278 (10): 8739–44. doi :10.1074/jbc.M211084200 PMID 12493755 . Shimizu Y, Iwai S, Hanaoka F, Sugasawa K (2003). "Xeroderma pigmentosum group C protein interacts physically and functionally with thymine DNA glycosylase" . EMBO J . 22 (1): 164–73. doi :10.1093/emboj/cdg016 . PMC 140069 PMID 12505994 . Chen D, Lucey MJ, Phoenix F, et al. (2003). "T:G mismatch-specific thymine-DNA glycosylase potentiates transcription of estrogen-regulated genes through direct interaction with estrogen receptor alpha" . J. Biol. Chem . 278 (40): 38586–92. doi :10.1074/jbc.M304286200 PMID 12874288 . Lehner B, Semple JI, Brown SE, et al. (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics . 83 (1): 153–67. doi :10.1016/S0888-7543(03)00235-0 . PMID 14667819 . Brandenberger R, Wei H, Zhang S, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol . 22 (6): 707–16. doi :10.1038/nbt971 . PMID 15146197 . S2CID 27764390 . Krześniak M, Butkiewicz D, Samojedny A, et al. (2005). "Polymorphisms in TDG and MGMT genes - epidemiological and functional study in lung cancer patients from Poland". Ann. Hum. Genet . 68 (Pt 4): 300–12. doi :10.1046/j.1529-8817.2004.00079.x . PMID 15225156 . S2CID 35293833 . He YF, Li BZ, Li Z, Liu P, Wang Y, Tang Q, Ding J, Jia Y, Chen Z, Li L, Sun Y, Li X, Dai Q, Song CX, Zhang K, He C, Xu GL (September 2011). "Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA" . Science . 333 (6047): 1303–7. Bibcode :2011Sci...333.1303H . doi :10.1126/science.1210944 . PMC 3462231 PMID 21817016 . Zhang L, Lu X, Lu J, Liang H, Dai Q, Xu GL, Luo C, Jiang H, He C (February 2012). "Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA" . Nature Chemical Biology . 8 (4): 328–30. doi :10.1038/nchembio.914 . PMC 3307914 PMID 22327402 . Xu J, Cortellino S, Tricarico R, Chang WC, Scher G, Devarajan K, Slifker M, Moore R, Bassi MR, Caretti E, Clapper M, Cooper H, Bellacosa A (September 2017). "Thymine DNA Glycosylase (TDG) is involved in the pathogenesis of intestinal tumors with reduced APC expression" . Oncotarget . 8 (52): 89988–89997. doi :10.18632/oncotarget.21219 . PMC 5685726 PMID 29163805 .
PDB 갤러리
1wyw : SMO1-콘크리트 티민 DNA 글리코실라아제의 결정구조
2d07 : SMO-3 수정 티민-DNA 글리코실라아제의 결정구조
