PCAF
PCAF
K(라이신) 아세틸전달효소 2B(KAT2B)로도 알려진 P300/CBP 관련인자(PCAF)는 p53과 관련된 인간 유전자 및 전사공활성제이다.
구조.
PCAF 의 몇개의 도메인은, 그 기능을 유효하게 하기 위해서, 개별적으로 또는 동시에 동작할 수 있습니다.PCAF는 별도의 아세틸전달효소 및 E3 유비퀴틴 리가아제 도메인과 다른 단백질과의 상호작용을 위한 브로모도메인을 가지고 있다.PCAF는 또한 자체 아세틸화 및 유비쿼티네이션 [5]사이트를 보유하고 있습니다.
기능.
CBP 및 p300은 c-준 및 아데노바이러스 온코프로틴 E1A를 포함하여 세포 성장 및/또는 분화에 관여하는 많은 배열 특이적 인자와 결합하는 대형 핵단백질이다.PCAF 유전자에 의해 암호화된 단백질은 p300/CBP와 관련된다.CBP 및 p300과 시험관내 및 생체내 결합 활성을 가지며, p300/CBP의 결합 부위에서 E1A와 경쟁한다.핵심 히스톤 및 뉴클레오솜 핵심 입자와 함께 히스톤 아세틸전달효소 활성을 가지며, 이는 이 단백질이 전사 [6]조절에 직접적인 역할을 한다는 것을 나타낸다.
규정
PCAF의 아세틸전달효소 활성과 세포 위치는 PCAF 자체의 아세틸화를 통해 조절된다.PCAF는 자동 아세틸화(자체 아세틸화)되거나 p300에 의해 생성될 수 있다.아세틸화는 핵으로의 이동을 유도하고 아세틸전달효소 [7]활성을 증가시킨다.PCAF는 HDAC3와 상호작용하고 HDAC3에 의해 탈아세틸화되어 PCAF 아세틸전달효소 활성의 감소와 세포질 [8]국재화를 초래한다.
단백질 상호작용
PCAF는 그 활동을 이끄는 수많은 단백질과 복합체를 형성한다.예를 들어 PCAF는 히스톤을 아세틸화하고 ATF4 표적 유전자의 전사를 촉진하기 위해 ATF에[9] 의해 모집된다.
대상
PCAF의 아세틸전달효소 활성에는 Fli1,[10] p53[11] 및 수많은 히스톤 잔류물과 같은 전사 인자를 포함한 다양한 단백질 표적이 있다.또한 Hdm2는 p53을 표적으로 하는 유비퀴틴 연결효소이며 [5]PCAF의 유비퀴틴 연결효소 활성의 표적이 되는 것으로 입증되었다.
상호 작용
PCAF는 다음 제품과 상호 작용하는 것으로 나타났습니다.
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레퍼런스
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- ^ a b c GRCm38: 앙상블 릴리즈 89: ENSMUSG000000708 - 앙상블, 2017년 5월
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외부 링크
- PCAF 단백질, 미국 국립 의학 도서관(MeSH)의 인간
- 누르사 C105
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