E3 ubiquitin-단백질 RAD18 은 인간에게 RAD18 유전자 에 의해 암호화 된 효소 다.[5] [6] [7]
함수 이 유전자에 의해 암호화된 단백질은 S. 세레비시아 DNA 손상 수리 단백질 Rad18과 매우 유사하다. 효모 Rad18은 손상된 DNA의 복제 후 복구에 필요한 유비퀴틴 콘주깅 효소인 Rad6와의 상호작용을 통해 기능한다. 이 단백질은 효모와 유사하게 보존된 약지 모티브를 통해 효모 Rad6 단백질의 인간 호몰로로그와 상호작용할 수 있다. 이 모티브의 돌연변이는 UV에 손상된 DNA의 불완전한 복제와 다수의 돌연변이에 대한 과민성을 초래한다.[7]
동물 모델 모델 유기체 는 RAD18 기능 연구에 사용되어 왔다.Rad18 이라고tm1a(EUCOMM)Wtsi 불리는 조건부 녹아웃 마우스 라인은 [8] 웰컴 트러스트 생거 연구소 (Wellcome Trust Sanger Institute)에서 관심 있는 과학자들에게 질병의 동물 모델을 생성하고 배포하기 위한 고처리 돌연변이 유발 프로젝트의 일환으로 생성되었다.[9] [10] [11] [12] [13] 래드18 이 부족한 생쥐는 생존력이나 생식력 에 큰 결함이 없어 수컷과 암컷이 표준화된 표현식 화면 을 통해 삭제 효과를 파악했다.[14] [15] [10] [16] [17]
25개의 테스트가 수행되었고 4개의 유의한 표현형식 이 보고되었다.[17]
인간 대장암 세포 라인의 녹아웃인 HCT116도 만들어졌다.[25]
상호작용 RAD18은 HLTF ,[26] UBE2B [5] [6] UBE2A 와 상호작용 하는 것으로 나타났다.[5] [6]
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