C1QBP
C1QBP
보체성분 1Q 서브성분 결합단백질인 미토콘드리아는 C1QBP [5][6][7]유전자에 의해 인체 내에서 암호화되는 단백질이다.
인간 보체 하위 성분 C1q는 혈청 보체 시스템의 첫 번째 성분을 생성하기 위해 C1r 및 C1s와 관련된다.이 유전자에 의해 암호화된 단백질은 C1q 분자의 구상 머리에 결합하고 C1 활성화를 억제하는 것으로 알려져 있다.이 단백질은 또한 히알루론산 결합 [7]단백질과 함께 사전 mRNA 스플라이싱 인자 SF2의 p32 서브유닛으로 확인되었다.
단백질 서브유닛
C1QBP는 길이 282개의 아미노산이며, N말단 73개의 아미노산 잔기를 분해하여 성숙한 C1QBP를 생성한다.C1QBP는 환원 및 비환원 조건 하에서 SDS-PAGE 겔에서 33kDa 내외의 단량체로 나타나지만 크기-제외 크로마토그래피(겔 여과)[8]에서는 트리머로 이동한다.
단백질 구조
C1QBP의 2.25Ω 분해능 결정 구조는 대칭을 나타내는 호모트리머 고리를 보여준다.개별 서브유닛은 비공유 상호 작용에 의해 함께 유지되며, 직경 20Ω의 중심 공동을 가진 도넛 모양의 4차 구조를 형성합니다.C1QBP의 각 서브유닛은 7개의 β스트랜드(β1~β7)와 3개의 α헬리시(α1~α3)를 가진다.C1QBP는 용해면에 음전하를 띠는 반면 막면은 [9]양전하를 띠게 된다.
상호 작용
C1QBP는 단백질인산화효소 D1,[10] BAT2,[11] PRKCD,[10] PKC[10] 알파 및 단백질인산화효소 [10]Mζ과 상호작용하는 것으로 나타났다. C1QBP의 다른 상호작용 파트너로는 박테리아,[12] 바이러스, 플라스모듐 팔시파룸 [14]등의 병원균에서 단백질 도메인이 있다.피브리노겐, FXII 및 HK를 포함한 혈장 단백질은 아연 의존적인 방식으로 C1QBP와 상호작용하는 것으로 입증되었다.[15][16]최근 종양 호밍 펩타이드인 LyP-1(CGNKRTRGC)은 종양 발현 [17]세포에서 C1QBP에 선택적으로 결합하는 것으로 나타났다.
레퍼런스
- ^ a b c GRCh38: 앙상블 릴리즈 89: ENSG00000108561 - 앙상블, 2017년 5월
- ^ a b c GRCm38: 앙상블 릴리즈 89: ENSMUSG000018446 - 앙상블, 2017년 5월
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Deb TB, Datta K (March 1996). "Molecular cloning of human fibroblast hyaluronic acid-binding protein confirms its identity with P-32, a protein co-purified with splicing factor SF2. Hyaluronic acid-binding protein as P-32 protein, co-purified with splicing factor SF2". J Biol Chem. 271 (4): 2206–12. doi:10.1074/jbc.271.4.2206. PMID 8567680.
- ^ Ghebrehiwet B, Lim BL, Peerschke EI, Willis AC, Reid KB (June 1994). "Isolation, cDNA cloning, and overexpression of a 33-kD cell surface glycoprotein that binds to the globular "heads" of C1q". J Exp Med. 179 (6): 1809–21. doi:10.1084/jem.179.6.1809. PMC 2191527. PMID 8195709.
- ^ a b "Entrez Gene: C1QBP complement component 1, q subcomponent binding protein".
- ^ Jiang, Jianzhong (1999). "rystal structure of human p32, a doughnut-shaped acidic mitochondrial matrix protein". PNAS. 96 (7): 3572–3577. Bibcode:1999PNAS...96.3572J. doi:10.1073/pnas.96.7.3572. PMC 22335. PMID 10097078.
- ^ Jiang, Jianzhong (1999). "rystal structure of human p32, a doughnut-shaped acidic mitochondrial matrix protein". PNAS. 96 (7): 3572–3577. Bibcode:1999PNAS...96.3572J. doi:10.1073/pnas.96.7.3572. PMC 22335. PMID 10097078.
- ^ a b c d Storz, P; Hausser A; Link G; Dedio J; Ghebrehiwet B; Pfizenmaier K; Johannes F J (August 2000). "Protein kinase C [micro] is regulated by the multifunctional chaperon protein p32". J. Biol. Chem. UNITED STATES. 275 (32): 24601–7. doi:10.1074/jbc.M002964200. ISSN 0021-9258. PMID 10831594.
- ^ Lehner, Ben; Semple Jennifer I; Brown Stephanie E; Counsell Damian; Campbell R Duncan; Sanderson Christopher M (January 2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics. United States. 83 (1): 153–67. doi:10.1016/S0888-7543(03)00235-0. ISSN 0888-7543. PMID 14667819.
- ^ Braun, Laurence; Ghebrehiwet, Berhane; Cossart, Pascale (2000). "gC1q-R/p32, a C1q-binding protein, is a receptor for the InlB invasion protein of Listeria monocytogenes". The EMBO Journal. 19 (7): 1458–1466. doi:10.1093/emboj/19.7.1458. PMC 310215. PMID 10747014.
- ^ Kittlesen, David J.; Chianese-Bullock, Kimberly A.; Yao, Zhi Q; Braciale, Thomas J.; Hahn, Young S. (2000). "Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation". J Clin Invest. 106 (10): 1239–1249. doi:10.1172/jci10323. PMC 381434. PMID 11086025.
- ^ Magallón-Tejada, Ariel (2016). "Cytoadhesion to gC1qR through Plasmodium falciparum Erythrocyte Membrane Protein 1 in Severe Malaria". PLOS Pathog. 12 (11): e1006011. doi:10.1371/journal.ppat.1006011. PMC 5106025. PMID 27835682.
- ^ Pathak, Monika; Kaira, Bubacarr G.; Slater, Alexandre; Emsley, Jonas (2018). "Cell Receptor and Cofactor Interactions of the Contact Activation System and Factor XI". Frontiers in Medicine. 5: 66. doi:10.3389/fmed.2018.00066. PMC 5871670. PMID 29619369.
- ^ Peerschke, EI; Bayer, AS; Ghebrehiwet, B; Xiong, YQ (2006). "gC1qR/p33 blockade reduces Staphylococcus aureus colonization of target tissues in an animal model of infective endocarditis". Infect Immun. 74 (8): 4418–4423. doi:10.1128/iai.01794-05. PMC 1539591. PMID 16861627.
- ^ Paasonen, Lauri; Sharma, Shweta; Braun, Gary B.; Kotamraju, Venkata R.; Chung, Thomas D.Y.; She, Zhigang; Sugahara, Kazuki N; Yliperttula, Marjo; Wu, Bainan; Pellecchia, Maurizio; Ruoslahti, Erkki; Teesalu, Tambet (2017). "New p32/gC1qR Ligands for Targeted Tumor Drug Delivery". ChemBioChem. 17 (7): 570–575. doi:10.1002/cbic.201500564. PMC 5433940. PMID 26895508.
추가 정보
- Krainer AR, Mayeda A, Kozak D, Binns G (1991). "Functional expression of cloned human splicing factor SF2: homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators". Cell. 66 (2): 383–94. doi:10.1016/0092-8674(91)90627-B. PMID 1830244. S2CID 24119556.
- Busby TF, Ingham KC (1990). "NH2-terminal calcium-binding domain of human complement C1s- mediates the interaction of C1r- with C1q". Biochemistry. 29 (19): 4613–8. doi:10.1021/bi00471a016. PMID 2372546.
- Fridell RA, Harding LS, Bogerd HP, Cullen BR (1995). "Identification of a novel human zinc finger protein that specifically interacts with the activation domain of lentiviral Tat proteins". Virology. 209 (2): 347–57. doi:10.1006/viro.1995.1266. PMID 7778269.
- Luo Y, Yu H, Peterlin BM (1994). "Cellular protein modulates effects of human immunodeficiency virus type 1 Rev". J. Virol. 68 (6): 3850–6. doi:10.1128/JVI.68.6.3850-3856.1994. PMC 236890. PMID 8189522.
- Honoré B, Madsen P, Rasmussen HH, et al. (1994). "Cloning and expression of a cDNA covering the complete coding region of the P32 subunit of human pre-mRNA splicing factor SF2". Gene. 134 (2): 283–7. doi:10.1016/0378-1119(93)90108-F. PMID 8262387.
- Tange TO, Jensen TH, Kjems J (1996). "In vitro interaction between human immunodeficiency virus type 1 Rev protein and splicing factor ASF/SF2-associated protein, p32". J. Biol. Chem. 271 (17): 10066–72. doi:10.1074/jbc.271.17.10066. PMID 8626563.
- Guo N, Weremowicz S, Lynch N, et al. (1997). "Assignment of C1QBP encoding the C1q globular domain binding protein (gC1q-R) to human chromosome 17 band p13.3 by in situ hybridization". Cytogenet. Cell Genet. 77 (3–4): 283–4. doi:10.1159/000134598. PMID 9284938.
- Majumdar M, Datta K (1998). "Assignment of cDNA encoding hyaluronic acid-binding protein 1 to human chromosome 17p12-p13". Genomics. 51 (3): 476–7. doi:10.1006/geno.1998.5364. PMID 9721222.
- Petersen-Mahrt SK, Estmer C, Ohrmalm C, et al. (1999). "The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation". EMBO J. 18 (4): 1014–24. doi:10.1093/emboj/18.4.1014. PMC 1171193. PMID 10022843.
- Jiang J, Zhang Y, Krainer AR, Xu RM (1999). "Crystal structure of human p32, a doughnut-shaped acidic mitochondrial matrix protein". Proc. Natl. Acad. Sci. U.S.A. 96 (7): 3572–7. Bibcode:1999PNAS...96.3572J. doi:10.1073/pnas.96.7.3572. PMC 22335. PMID 10097078.
- Braun L, Ghebrehiwet B, Cossart P (2000). "gC1q-R/p32, a C1q-binding protein, is a receptor for the InlB invasion protein of Listeria monocytogenes". EMBO J. 19 (7): 1458–66. doi:10.1093/emboj/19.7.1458. PMC 310215. PMID 10747014.
- Beatch MD, Hobman TC (2000). "Rubella virus capsid associates with host cell protein p32 and localizes to mitochondria". J. Virol. 74 (12): 5569–76. doi:10.1128/JVI.74.12.5569-5576.2000. PMC 112044. PMID 10823864.
- Storz P, Hausser A, Link G, et al. (2000). "Protein kinase C [micro] is regulated by the multifunctional chaperon protein p32". J. Biol. Chem. 275 (32): 24601–7. doi:10.1074/jbc.M002964200. PMID 10831594.
- Tye AJ, Ghebrehiwet B, Guo N, et al. (2001). "The human gC1qR/p32 gene, C1qBP. Genomic organization and promoter analysis". J. Biol. Chem. 276 (20): 17069–75. doi:10.1074/jbc.M009064200. PMID 11278463.
- Schaerer MT, Kannenberg K, Hunziker P, et al. (2001). "Interaction between GABA(A) receptor beta subunits and the multifunctional protein gC1q-R". J. Biol. Chem. 276 (28): 26597–604. doi:10.1074/jbc.M102534200. PMID 11350968.
- Kobayashi M, Hanai R (2001). "M phase-specific association of human topoisomerase IIIbeta with chromosomes". Biochem. Biophys. Res. Commun. 287 (1): 282–7. doi:10.1006/bbrc.2001.5580. PMID 11549288.
- Rozanov DV, Ghebrehiwet B, Postnova TI, et al. (2002). "The hemopexin-like C-terminal domain of membrane type 1 matrix metalloproteinase regulates proteolysis of a multifunctional protein, gC1qR". J. Biol. Chem. 277 (11): 9318–25. doi:10.1074/jbc.M110711200. PMID 11773076.
- Majumdar M, Meenakshi J, Goswami SK, Datta K (2002). "Hyaluronan binding protein 1 (HABP1)/C1QBP/p32 is an endogenous substrate for MAP kinase and is translocated to the nucleus upon mitogenic stimulation". Biochem. Biophys. Res. Commun. 291 (4): 829–37. doi:10.1006/bbrc.2002.6491. PMID 11866440.
외부 링크
- UCSC Genome Browser의 인간 C1QBP 게놈 위치 및 C1QBP 유전자 세부 정보 페이지.
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