원산지 인식 복합체 2단위 는 인간의 ORC2 (ORC2L) 유전자 에 의해 암호화된 단백질 이다.[5] [6]
함수 원산지 인식 콤플렉스 (ORC)는 진핵세포에서 DNA 복제를 시작하는 데 필수적인 6개의 서브유닛 단백질 콤플렉스다.효모에 대한 연구는 ORC가 복제의 기원에 특별히 결합하고 있으며 Cdc6 와 Mcm 단백질과 같은 추가 개시 인자의 조립을 위한 플랫폼 역할을 한다는 것을 입증했다. 이 유전자에 의해 인코딩된 단백질은 ORC 복합체의 서브 유닛이다. 이 단백질은 ORC3 , ORC4 , ORC5 와 함께 핵심 복합체를 형성한다. 또한 DNA 복제의 시작에 중요한 것으로 알려진 단백질인 CDC45L , MCM10과도 상호작용한다. 이 단백질은 인간 세포에서 엡스타인-바르 바이러스의 복제 원점과 특별히 연관되어 있다는 것이 증명되었으며, 복제의 바이러스 원점에서 DNA 복제가 필요한 것으로 생각된다.[6]
상호작용 ORC2는 다음과 상호작용 하는 것으로 나타났다.
CDC6 ,[7] [8] DBF4 ,[7] MCM10 ,[9] MCM2 [7] MCM4 ,[7] MCM5 ,[7] MCM6 ,[7] MCM7 ,[7] ORC1 ,[7] [10] [11] [12] ORC3 ,[7] [11] [13] [14] [15] ORC4 ,[7] [11] [13] [16] ORC5 ,[7] [11] [13] [17] ORC6 [7] [11] 복제 단백질 A1 .[7]
참조 ^ a b c GRCh38: 앙상블 릴리스 89: ENSG00000115942 - 앙상블 , 2017년 5월^ a b c GRCm38: 앙상블 릴리스 89: ENSMUSG000026037 - 앙상블 , 2017년 5월^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Takahara K, Bong M, Brevard R, Eddy RL, Haley LL, Sait SJ, Shows TB, Hoffman GG, Greenspan DS (October 1996). "Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L". Genomics . 31 (1): 119–122. doi :10.1006/geno.1996.0018 . PMID 8808289 . ^ a b "Entrez Gene: ORC2L origin recognition complex, subunit 2-like (yeast)" .^ a b c d e f g h i j k l m Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol . 327 (1): 111–28. doi :10.1016/s0022-2836(03)00079-2 . PMID 12614612 . ^ Méndez J, Stillman B (November 2000). "Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis" . Mol. Cell. Biol . 20 (22): 8602–12. doi :10.1128/mcb.20.22.8602-8612.2000 . PMC 102165 PMID 11046155 . ^ Izumi M, Yanagi K, Mizuno T, Yokoi M, Kawasaki Y, Moon KY, Hurwitz J, Yatagai F, Hanaoka F (December 2000). "The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase" . Nucleic Acids Res . 28 (23): 4769–77. doi :10.1093/nar/28.23.4769 . PMC 115166 PMID 11095689 . ^ Fujita M, Ishimi Y, Nakamura H, Kiyono T, Tsurumi T (March 2002). "Nuclear organization of DNA replication initiation proteins in mammalian cells" . J. Biol. Chem . 277 (12): 10354–61. doi :10.1074/jbc.M111398200 PMID 11779870 . ^ a b c d e Vashee S, Simancek P, Challberg MD, Kelly TJ (July 2001). "Assembly of the human origin recognition complex" . J. Biol. Chem . 276 (28): 26666–73. doi :10.1074/jbc.M102493200 PMID 11323433 . ^ Méndez J, Zou-Yang XH, Kim SY, Hidaka M, Tansey WP, Stillman B (March 2002). "Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication" . Mol. Cell . 9 (3): 481–91. doi :10.1016/s1097-2765(02)00467-7 PMID 11931757 . ^ a b c Dhar SK, Delmolino L, Dutta A (August 2001). "Architecture of the human origin recognition complex" . J. Biol. Chem . 276 (31): 29067–71. doi :10.1074/jbc.M103078200 PMID 11395502 . ^ Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ (May 2007). "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage". Science . 316 (5828): 1160–6. doi :10.1126/science.1140321 . PMID 17525332 . S2CID 16648052 . ^ Pinto S, Quintana DG, Smith P, Mihalek RM, Hou ZH, Boynton S, Jones CJ, Hendricks M, Velinzon K, Wohlschlegel JA, Austin RJ, Lane WS, Tully T, Dutta A (May 1999). "latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant" . Neuron . 23 (1): 45–54. doi :10.1016/s0896-6273(00)80752-7 PMID 10402192 . S2CID 781511 . ^ Quintana DG, Hou Zh, Thome KC, Hendricks M, Saha P, Dutta A (November 1997). "Identification of HsORC4, a member of the human origin of replication recognition complex" . J. Biol. Chem . 272 (45): 28247–51. doi :10.1074/jbc.272.45.28247 PMID 9353276 . ^ Quintana DG, Thome KC, Hou Zh, Ligon AH, Morton CC, Dutta A (October 1998). "ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases" . J. Biol. Chem . 273 (42): 27137–45. doi :10.1074/jbc.273.42.27137 PMID 9765232 . 추가 읽기 Gavin KA, Hidaka M, Stillman B (1996). "Conserved initiator proteins in eukaryotes". Science . 270 (5242): 1667–1671. doi :10.1126/science.270.5242.1667 . PMID 7502077 . S2CID 43633493 . Saha P, Thome KC, Yamaguchi R, Hou Z, Weremowicz S, Dutta A (1998). "The human homolog of Saccharomyces cerevisiae CDC45" . J. Biol. Chem . 273 (29): 18205–18209. doi :10.1074/jbc.273.29.18205 PMID 9660782 . Ritzi M, Baack M, Musahl C, Romanowski P, Laskey RA, Knippers R (1998). "Human minichromosome maintenance proteins and human origin recognition complex 2 protein on chromatin" . J. Biol. Chem . 273 (38): 24543–24549. doi :10.1074/jbc.273.38.24543 PMID 9733749 . Pinto S, Quintana DG, Smith P, Mihalek RM, Hou ZH, Boynton S, Jones CJ, Hendricks M, Velinzon K, Wohlschlegel JA, Austin RJ, Lane WS, Tully T, Dutta A (1999). "latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant" . Neuron . 23 (1): 45–54. doi :10.1016/S0896-6273(00)80752-7 PMID 10402192 . S2CID 781511 . Jiang W, McDonald D, Hope TJ, Hunter T (1999). "Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication" . EMBO J . 18 (20): 5703–5713. doi :10.1093/emboj/18.20.5703 . PMC 1171637 PMID 10523313 . Natale DA, Li CJ, Sun WH, DePamphilis ML (2000). "Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals" . EMBO J . 19 (11): 2728–2738. doi :10.1093/emboj/19.11.2728 . PMC 212765 PMID 10835370 . Thome KC, Dhar SK, Quintana DG, Delmolino L, Shahsafaei A, Dutta A (2001). "Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins" . J. Biol. Chem . 275 (45): 35233–35241. doi :10.1074/jbc.M005765200 PMID 10954718 . Izumi M, Yanagi K, Mizuno T, Yokoi M, Kawasaki Y, Moon KY, Hurwitz J, Yatagai F, Hanaoka F (2001). "The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase" . Nucleic Acids Res . 28 (23): 4769–4777. doi :10.1093/nar/28.23.4769 . PMC 115166 PMID 11095689 . Vashee S, Simancek P, Challberg MD, Kelly TJ (2001). "Assembly of the human origin recognition complex" . J. Biol. Chem . 276 (28): 26666–26673. doi :10.1074/jbc.M102493200 PMID 11323433 . Dhar SK, Delmolino L, Dutta A (2001). "Architecture of the human origin recognition complex" . J. Biol. Chem . 276 (31): 29067–29071. doi :10.1074/jbc.M103078200 PMID 11395502 . Dhar SK, Yoshida K, Machida Y, Khaira P, Chaudhuri B, Wohlschlegel JA, Leffak M, Yates J, Dutta A (2001). "Replication from oriP of Epstein-Barr virus requires human ORC and is inhibited by geminin" . Cell . 106 (3): 287–296. doi :10.1016/S0092-8674(01)00458-5 PMID 11509178 . S2CID 12479646 . Izumi M, Yatagai F, Hanaoka F (2002). "Cell cycle-dependent proteolysis and phosphorylation of human Mcm10" . J. Biol. Chem . 276 (51): 48526–31. doi :10.1074/jbc.M107190200 PMID 11602595 . Fujita M, Ishimi Y, Nakamura H, Kiyono T, Tsurumi T (2002). "Nuclear organization of DNA replication initiation proteins in mammalian cells" . J. Biol. Chem . 277 (12): 10354–10361. doi :10.1074/jbc.M111398200 PMID 11779870 . Schaarschmidt D, Ladenburger EM, Keller C, Knippers R (2002). "Human Mcm proteins at a replication origin during the G1 to S phase transition" . Nucleic Acids Res . 30 (19): 4176–4185. doi :10.1093/nar/gkf532 . PMC 140533 PMID 12364596 . Matheos D, Ruiz MT, Price GB, Zannis-Hadjopoulos M (2002). "Ku antigen, an origin-specific binding protein that associates with replication proteins, is required for mammalian DNA replication". Biochim. Biophys. Acta . 1578 (1–3): 59–72. doi :10.1016/S0167-4781(02)00497-9 . PMID 12393188 . Kneissl M, Pütter V, Szalay AA, Grummt F (2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol . 327 (1): 111–128. doi :10.1016/S0022-2836(03)00079-2 . PMID 12614612 .
외부 링크 PDBe-KB 는 휴먼 오리진 인식 콤플렉스 2단위(ORC2)에 대해 PDB에서 이용할 수 있는 모든 구조 정보에 대한 개요를 제공한다. 
