BUB1B

BUB1B
사용 가능한 구조
PDB	Ortholog 검색: PDBe RCSB
PDB ID 코드 목록
	2WVI, 3SI5, 4GGD, 5JA
식별자
에일리어스	BUB1B, BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBR1, BUB1 유사분열체크포인트세린/트레오닌인산화효소B
외부 ID	OMIM : 602860 MGI : 1333889 HomoloGene : 933 GenCard : BUB1B
유전자 위치(인간)
크르.	15번 염색체(인간)
끝.	40,221,123 bp
유전자 위치(마우스)
크르.	2번 염색체(마우스)
끝.	118,472,072 bp
RNA발현패턴
	상단 표시 위치:
	이차 난모세포; ; 태아; ; 신경절 융기; ; 골수; ; 결석골; ; 골수 세포; ; 흉선; ; 직장; ; 부록; ; 자궁내막간질세포;
	추가 참조 표현식 데이터
	추가 참조 표현식 데이터
유전자 존재론
분자 함수	단백질세린/트레오닌인산화효소활성; ATP 결합; GO:0001948 단백질 결합; 키나아제활성; 뉴클레오티드 결합; 트랜스페라아제 활성; 단백질인산화효소활성;
셀룰러 컴포넌트	키네토코어; 핵; 세포골격; 외부 운동체; 미소관 조직 센터; 세포질핵주위영역; 염색체, 중심체 영역; 아나phase-metric 착화체; 염색체; 세포질; 방추체 중간대; 세포; 스핀들;
생물학적 과정	염색체로의 단백질 국재, 중심체 영역; 아나phase-complex-의존성 이화 과정; 유사분열세포주기체크포인트시그널링; 단백질인산화; GO:0051323 유사분열세포주기의 메타기/아나기 전이; 세포 주기; 아포토시스 과정; 인산화; 세포 분열; 세포군 증식; 키네토코어로의 단백질 국재화; 유사분열 스핀들 어셈블리 체크포인트 시그널링; 감수성 자매 염색체 응집, 중심체; GO:0007067 유사분열세포 사이클; 유사분열세포주기상전이조절; 유비퀴틴의존성 단백질 이화 과정;
	출처:아미고 / QuickGO
맞춤법
	701
	12236
	ENSG00000156970
	ENSMUSG000040084
	O60566
	Q9Z1S0
	NM_001211
	NM_009773
	NP_001202
	NP_033903
	위키데이터
인간 보기/편집	마우스 표시/편집

유사분열체크포인트세린/트레오닌단백질인산화효소 BUB1베타는 BUB1B유전자에 ^[5]의해 인체에서 코드되는 효소이다.

기능.

이 유전자는 방추체크포인트 기능과 염색체 ^[6]분리에 관여하는 키나제를 코드한다.단백질은 키네토코어에 국소화되어 아나파아제 촉진 복합체/시클로좀(APC/C)을 억제하는 역할을 하며 아나파아제 시작을 지연시키고 적절한 염색체 분리를 보장한다.스핀들 체크포인트 기능의 장애는 여러 형태의 ^[7]암에서 발견되어 왔다.

생쥐에서 BubR1의 발현이 증가하면 건강한 ^[8]수명이 연장됩니다.

DNA복구

화학요법과 방사선요법의 조합인 화학방사선요법(CRT)은 다양한 암 치료에 사용된다.CRT는 암세포의 DNA에 손상을 유발하는 역할을 한다.방광암 종양 샘플은 치료 전 환자와 종양이 ^[9]재발했을 때 CRT 치료 후 동일한 환자로부터 채취되었다.CRT-재귀 셀에서 BUB1B 발현 수준이 증가했습니다.이 증가된 발현은 CRT에 의해 야기된 것과 같은 DNA 손상을 부정확하게 복구하는 대체 비호몰로지 엔드 결합(A-NHEJ)이라고 불리는 부정확한 DNA 복구 과정을 용이하게 하기 위해 고려되었다.이 부정확한 복구는 CRT ^[9]내성에 대한 돌연변이를 포함하여 종양에 추가적인 돌연변이를 일으킬 수 있습니다.

상호 작용

BUB1B는 다음과 상호작용하는 것으로 나타났습니다.

AP2B1,^[10]
BRCA2^[11]
BUB3,^[10]^[12]^[13]
CDC20,^[14]^[15]^[16]^[17]^[18]^[19]
HDAC1,^[20]
MAD2L1 ^[14]및
SNCG^[21]

레퍼런스

^ ^a ^b ^c GRCh38: 앙상블 릴리즈 89: ENSG00000156970 - 앙상블, 2017년 5월
^ ^a ^b ^c GRCm38: 앙상블 릴리즈 89: ENSMUSG000040084 - 앙상블, 2017년 5월
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Davenport JW, Fernandes ER, Harris LD, Neale GA, Goorha R (March 1999). "The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner". Genomics. 55 (1): 113–7. doi:10.1006/geno.1998.5629. PMID 9889005.
^ Guo Y, Kim C, Ahmad S, Zhang J, Mao Y (July 2012). "CENP-E--dependent BubR1 autophosphorylation enhances chromosome alignment and the mitotic checkpoint". J. Cell Biol. 198 (2): 205–217. doi:10.1083/jcb.201202152. PMC 3410423. PMID 22801780.
^ "Entrez Gene: BUB1B BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast)".
^
Baker DJ, Dawlaty MM, Wijshake T, Jeganathan KB, Malureanu L, van Ree JH, Crespo-Diaz R, Reyes S, Seaburg L, Shapiro V, Behfar A, Terzic A, van de Sluis B, van Deursen JM (December 2012). "Increased expression of BubR1 protects against aneuploidy and cancer and extends healthy lifespan". Nat. Cell Biol. 15 (1): 96–102. doi:10.1038/ncb2643. PMC 3707109. PMID 23242215.
- "Mayo Clinic Study Unmasks Regulator of Healthy Life Span". Mayo Clinic. December 17, 2012.
^ ^a ^b Komura K, Inamoto T, Tsujino T, Matsui Y, Konuma T, Nishimura K, et al. (October 2021). "Increased BUB1B/BUBR1 expression contributes to aberrant DNA repair activity leading to resistance to DNA-damaging agents". Oncogene. 40 (43): 6210–6222. doi:10.1038/s41388-021-02021-y. PMC 8553621. PMID 34545188.
^ ^a ^b Cayrol C, Cougoule C, Wright M (November 2002). "The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase BubR1". Biochem. Biophys. Res. Commun. 298 (5): 720–30. doi:10.1016/S0006-291X(02)02522-6. PMID 12419313.
^ Futamura M, Arakawa H, Matsuda K, Katagiri T, Saji S, Miki Y, Nakamura Y (March 2000). "Potential role of BRCA2 in a mitotic checkpoint after phosphorylation by hBUBR1". Cancer Res. 60 (6): 1531–5. PMID 10749118.
^ Hoyt MA (September 2001). "A new view of the spindle checkpoint". J. Cell Biol. 154 (5): 909–11. doi:10.1083/jcb.200108010. PMC 2196198. PMID 11535614.
^ Taylor SS, Ha E, McKeon F (July 1998). "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase". J. Cell Biol. 142 (1): 1–11. doi:10.1083/jcb.142.1.1. PMC 2133037. PMID 9660858.
^ ^a ^b Sudakin V, Chan GK, Yen TJ (September 2001). "Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2". J. Cell Biol. 154 (5): 925–36. doi:10.1083/jcb.200102093. PMC 2196190. PMID 11535616.
^ Fang G (March 2002). "Checkpoint protein BubR1 acts synergistically with Mad2 to inhibit anaphase-promoting complex". Mol. Biol. Cell. 13 (3): 755–66. doi:10.1091/mbc.01-09-0437. PMC 99596. PMID 11907259.
^ Wu H, Lan Z, Li W, Wu S, Weinstein J, Sakamoto KM, Dai W (September 2000). "p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase". Oncogene. 19 (40): 4557–62. doi:10.1038/sj.onc.1203803. PMID 11030144.
^ Kallio MJ, Beardmore VA, Weinstein J, Gorbsky GJ (September 2002). "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". J. Cell Biol. 158 (5): 841–7. doi:10.1083/jcb.200201135. PMC 2173153. PMID 12196507.
^ Skoufias DA, Andreassen PR, Lacroix FB, Wilson L, Margolis RL (April 2001). "Mammalian mad2 and bub1/bubR1 recognize distinct spindle-attachment and kinetochore-tension checkpoints". Proc. Natl. Acad. Sci. U.S.A. 98 (8): 4492–7. Bibcode:2001PNAS...98.4492S. doi:10.1073/pnas.081076898. PMC 31862. PMID 11274370.
^ Nilsson J, Yekezare M, Minshull J, Pines J (December 2008). "The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction". Nat. Cell Biol. 10 (12): 1411–20. doi:10.1038/ncb1799. PMC 2635557. PMID 18997788.
^ Yoon YM, Baek KH, Jeong SJ, Shin HJ, Ha GH, Jeon AH, Hwang SG, Chun JS, Lee CW (September 2004). "WD repeat-containing mitotic checkpoint proteins act as transcriptional repressors during interphase". FEBS Lett. 575 (1–3): 23–9. doi:10.1016/j.febslet.2004.07.089. ISSN 0014-5793. PMID 15388328. S2CID 21762011.
^ Gupta A, Inaba S, Wong OK, Fang G, Liu J (October 2003). "Breast cancer-specific gene 1 interacts with the mitotic checkpoint kinase BubR1". Oncogene. 22 (48): 7593–9. doi:10.1038/sj.onc.1206880. PMID 14576821.

추가 정보

Posas F, Saito H (1998). "Activation of the yeast SSK2 MAP kinase kinase kinase by the SSK1 two-component response regulator". EMBO J. 17 (5): 1385–94. doi:10.1093/emboj/17.5.1385. PMC 1170486. PMID 9482735.
Cahill DP, Lengauer C, Yu J, Riggins GJ, Willson JK, Markowitz SD, Kinzler KW, Vogelstein B (1998). "Mutations of mitotic checkpoint genes in human cancers". Nature. 392 (6673): 300–3. Bibcode:1998Natur.392..300C. doi:10.1038/32688. PMID 9521327. S2CID 4416376.
Donadelli R, Benatti L, Remuzzi A, Morigi M, Gullans SR, Benigni A, Remuzzi G, Noris M (1998). "Identification of a novel gene--SSK1--in human endothelial cells exposed to shear stress". Biochem. Biophys. Res. Commun. 246 (3): 881–7. doi:10.1006/bbrc.1998.8713. PMID 9618306.
Chan GK, Schaar BT, Yen TJ (1998). "Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1". J. Cell Biol. 143 (1): 49–63. doi:10.1083/jcb.143.1.49. PMC 2132809. PMID 9763420.
Jablonski SA, Chan GK, Cooke CA, Earnshaw WC, Yen TJ (1999). "The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosis". Chromosoma. 107 (6–7): 386–96. doi:10.1007/s004120050322. PMID 9914370. S2CID 13928165.
Cahill DP, da Costa LT, Carson-Walter EB, Kinzler KW, Vogelstein B, Lengauer C (1999). "Characterization of MAD2B and other mitotic spindle checkpoint genes". Genomics. 58 (2): 181–7. doi:10.1006/geno.1999.5831. PMID 10366450.
Chan GK, Jablonski SA, Sudakin V, Hittle JC, Yen TJ (1999). "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC". J. Cell Biol. 146 (5): 941–54. doi:10.1083/jcb.146.5.941. PMC 2169490. PMID 10477750.
Li W, Lan Z, Wu H, Wu S, Meadows J, Chen J, Zhu V, Dai W (2000). "BUBR1 phosphorylation is regulated during mitotic checkpoint activation". Cell Growth Differ. 10 (11): 769–75. PMID 10593653.
Saffery R, Irvine DV, Griffiths B, Kalitsis P, Choo KH (2001). "Components of the human spindle checkpoint control mechanism localize specifically to the active centromere on dicentric chromosomes". Hum. Genet. 107 (4): 376–84. doi:10.1007/s004390000386. PMID 11129339. S2CID 38578162.
Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
Kaplan KB, Burds AA, Swedlow JR, Bekir SS, Sorger PK, Näthke IS (2001). "A role for the Adenomatous Polyposis Coli protein in chromosome segregation". Nat. Cell Biol. 3 (4): 429–32. doi:10.1038/35070123. PMID 11283619. S2CID 12645435.
Tang Z, Bharadwaj R, Li B, Yu H (2001). "Mad2-Independent inhibition of APCCdc20 by the mitotic checkpoint protein BubR1". Dev. Cell. 1 (2): 227–37. doi:10.1016/S1534-5807(01)00019-3. PMID 11702782.
Shichiri M, Yoshinaga K, Hisatomi H, Sugihara K, Hirata Y (2002). "Genetic and epigenetic inactivation of mitotic checkpoint genes hBUB1 and hBUBR1 and their relationship to survival". Cancer Res. 62 (1): 13–7. PMID 11782350.
Taylor SS, Hussein D, Wang Y, Elderkin S, Morrow CJ (2002). "Kinetochore localisation and phosphorylation of the mitotic checkpoint components Bub1 and BubR1 are differentially regulated by spindle events in human cells". J. Cell Sci. 114 (Pt 24): 4385–95. doi:10.1242/jcs.114.24.4385. PMID 11792804.

외부 링크

UCSC Genome Browser의 인간 BUB1B 게놈 위치 및 BUB1B 유전자 세부 정보 페이지.

[refGRCh38Ensembl-1] GRCh38: 앙상블 릴리즈 89: ENSG00000156970 - 앙상블, 2017년 5월

[refGRCm38Ensembl-2] GRCm38: 앙상블 릴리즈 89: ENSMUSG000040084 - 앙상블, 2017년 5월

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9889005-5] Davenport JW, Fernandes ER, Harris LD, Neale GA, Goorha R (March 1999). "The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner". Genomics. 55 (1): 113–7. doi:10.1006/geno.1998.5629. PMID 9889005.

[pmid22801780-6] Guo Y, Kim C, Ahmad S, Zhang J, Mao Y (July 2012). "CENP-E--dependent BubR1 autophosphorylation enhances chromosome alignment and the mitotic checkpoint". J. Cell Biol. 198 (2): 205–217. doi:10.1083/jcb.201202152. PMC 3410423. PMID 22801780.

[entrez-7] "Entrez Gene: BUB1B BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast)".

[pmid23242215-8] Baker DJ, Dawlaty MM, Wijshake T, Jeganathan KB, Malureanu L, van Ree JH, Crespo-Diaz R, Reyes S, Seaburg L, Shapiro V, Behfar A, Terzic A, van de Sluis B, van Deursen JM (December 2012). "Increased expression of BubR1 protects against aneuploidy and cancer and extends healthy lifespan". Nat. Cell Biol. 15 (1): 96–102. doi:10.1038/ncb2643. PMC 3707109. PMID 23242215.
"Mayo Clinic Study Unmasks Regulator of Healthy Life Span". Mayo Clinic. December 17, 2012.

[9] "Mayo Clinic Study Unmasks Regulator of Healthy Life Span". Mayo Clinic. December 17, 2012.

[Komura2021-9] Komura K, Inamoto T, Tsujino T, Matsui Y, Konuma T, Nishimura K, et al. (October 2021). "Increased BUB1B/BUBR1 expression contributes to aberrant DNA repair activity leading to resistance to DNA-damaging agents". Oncogene. 40 (43): 6210–6222. doi:10.1038/s41388-021-02021-y. PMC 8553621. PMID 34545188.

[pmid12419313-10] Cayrol C, Cougoule C, Wright M (November 2002). "The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase BubR1". Biochem. Biophys. Res. Commun. 298 (5): 720–30. doi:10.1016/S0006-291X(02)02522-6. PMID 12419313.

[pmid10749118-11] Futamura M, Arakawa H, Matsuda K, Katagiri T, Saji S, Miki Y, Nakamura Y (March 2000). "Potential role of BRCA2 in a mitotic checkpoint after phosphorylation by hBUBR1". Cancer Res. 60 (6): 1531–5. PMID 10749118.

[pmid11535614-12] Hoyt MA (September 2001). "A new view of the spindle checkpoint". J. Cell Biol. 154 (5): 909–11. doi:10.1083/jcb.200108010. PMC 2196198. PMID 11535614.

[pmid9660858-13] Taylor SS, Ha E, McKeon F (July 1998). "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase". J. Cell Biol. 142 (1): 1–11. doi:10.1083/jcb.142.1.1. PMC 2133037. PMID 9660858.

[pmid11535616-14] Sudakin V, Chan GK, Yen TJ (September 2001). "Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2". J. Cell Biol. 154 (5): 925–36. doi:10.1083/jcb.200102093. PMC 2196190. PMID 11535616.

[pmid11907259-15] Fang G (March 2002). "Checkpoint protein BubR1 acts synergistically with Mad2 to inhibit anaphase-promoting complex". Mol. Biol. Cell. 13 (3): 755–66. doi:10.1091/mbc.01-09-0437. PMC 99596. PMID 11907259.

[pmid11030144-16] Wu H, Lan Z, Li W, Wu S, Weinstein J, Sakamoto KM, Dai W (September 2000). "p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase". Oncogene. 19 (40): 4557–62. doi:10.1038/sj.onc.1203803. PMID 11030144.

[pmid12196507-17] Kallio MJ, Beardmore VA, Weinstein J, Gorbsky GJ (September 2002). "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". J. Cell Biol. 158 (5): 841–7. doi:10.1083/jcb.200201135. PMC 2173153. PMID 12196507.

[pmid11274370-18] Skoufias DA, Andreassen PR, Lacroix FB, Wilson L, Margolis RL (April 2001). "Mammalian mad2 and bub1/bubR1 recognize distinct spindle-attachment and kinetochore-tension checkpoints". Proc. Natl. Acad. Sci. U.S.A. 98 (8): 4492–7. Bibcode:2001PNAS...98.4492S. doi:10.1073/pnas.081076898. PMC 31862. PMID 11274370.

[pmid18997788-19] Nilsson J, Yekezare M, Minshull J, Pines J (December 2008). "The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction". Nat. Cell Biol. 10 (12): 1411–20. doi:10.1038/ncb1799. PMC 2635557. PMID 18997788.

[pmid15388328-20] Yoon YM, Baek KH, Jeong SJ, Shin HJ, Ha GH, Jeon AH, Hwang SG, Chun JS, Lee CW (September 2004). "WD repeat-containing mitotic checkpoint proteins act as transcriptional repressors during interphase". FEBS Lett. 575 (1–3): 23–9. doi:10.1016/j.febslet.2004.07.089. ISSN 0014-5793. PMID 15388328. S2CID 21762011.

[pmid14576821-21] Gupta A, Inaba S, Wong OK, Fang G, Liu J (October 2003). "Breast cancer-specific gene 1 interacts with the mitotic checkpoint kinase BubR1". Oncogene. 22 (48): 7593–9. doi:10.1038/sj.onc.1206880. PMID 14576821.

[1]

[2]

[3]

[4]

[5]

[6]

[7]

[8]

[9]

[10]

[11]

[12]

[13]

[14]

[15]

[16]

[17]

[18]

[19]

[20]

[21]

Search