NEK2
NEK2
세린/트레오닌-단백질인산화효소 Nek2는 NEK2 [5][6]유전자에 의해 사람에게서 코드되는 효소이다.
상호 작용
NEK2는 MAPK1[7] 및 NDC80과 [8][9]상호 작용하는 것으로 나타났습니다.유사분열세포에서의 중심체 분리 및 양극성 방추 형성 제어 및 감수세포에서의 염색질 응축 제어에 관여하는 단백질 키나제.CROCC, CEP250, NINL 등의 중심체 단백질을 인산화하여 중심체로부터의 변위를 초래함으로써 중심체 분리를 조절한다.NDC80의 인산화를 통해 유사분열 시 키네토코어 미세관 부착 안정성을 조절합니다.CDC20 및 MAD2L1의 인산화를 통한 유사분열 체크포인트 단백질 복합체 조절에 관여한다.HMGA2의 인산화를 통해 제1차 감수분열 중에 염색질 축합에 활성적 역할을 한다.인산화물:PPP1CC;SGOL1,NECAB3,NPM1.키네토코어에 대한 MAD2L1 및 센트로솜에 대한 NPM1의 국재화에 필수적이다.Isoform 1은 G1/S-arrested 세포에서 NEK11을 인산화 및 활성화한다.핵에 존재하지 않는 Isoform 2는 [유니프로트]하지 않는다.
레퍼런스
- ^ a b c GRCh38: 앙상블 릴리즈 89: ENSG00000117650 - 앙상블, 2017년 5월
- ^ a b c GRCm38: 앙상블 릴리즈 89: ENSMUSG000026622 - 앙상블, 2017년 5월
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Schultz SJ, Nigg EA (Feb 1994). "Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans". Cell Growth Differ. 4 (10): 821–30. PMID 8274451.
- ^ "Entrez Gene: NEK2 NIMA (never in mitosis gene a)-related kinase 2".
- ^ Lou Y, Xie W, Zhang DF, Yao JH, Luo ZF, Wang YZ, Shi YY, Yao XB (Aug 2004). "Nek2A specifies the centrosomal localization of Erk2". Biochem. Biophys. Res. Commun. 321 (2): 495–501. doi:10.1016/j.bbrc.2004.06.171. PMID 15358203.
- ^ Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH (Dec 2002). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". J. Biol. Chem. 277 (51): 49408–16. doi:10.1074/jbc.M207069200. PMID 12386167.
- ^ Chen Y, Sharp ZD, Lee WH (Sep 1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". J. Biol. Chem. 272 (38): 24081–7. doi:10.1074/jbc.272.38.24081. PMID 9295362.
추가 정보
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- Schultz SJ, Fry AM, Sütterlin C, Ried T, Nigg EA (1994). "Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans". Cell Growth Differ. 5 (6): 625–35. PMID 7522034.
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
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- Fry AM, Meraldi P, Nigg EA (1998). "A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators". EMBO J. 17 (2): 470–81. doi:10.1093/emboj/17.2.470. PMC 1170398. PMID 9430639.
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- Fry AM, Arnaud L, Nigg EA (1999). "Activity of the human centrosomal kinase, Nek2, depends on an unusual leucine zipper dimerization motif". J. Biol. Chem. 274 (23): 16304–10. doi:10.1074/jbc.274.23.16304. PMID 10347187.
- Schutte BC, Bjork BC, Coppage KB, Malik MI, Gregory SG, Scott DJ, Brentzell LM, Watanabe Y, Dixon MJ, Murray JC (2000). "A preliminary gene map for the Van der Woude syndrome critical region derived from 900 kb of genomic sequence at 1q32-q41". Genome Res. 10 (1): 81–94. doi:10.1101/gr.10.1.81 (inactive 28 February 2022). PMC 310500. PMID 10645953.
{{cite journal}}: CS1 유지 : 2022년 2월 현재 DOI 비활성화 (링크) - Helps NR, Luo X, Barker HM, Cohen PT (2001). "NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1". Biochem. J. 349 (Pt 2): 509–18. doi:10.1042/0264-6021:3490509. PMC 1221174. PMID 10880350.
- Hames RS, Fry AM (2002). "Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis". Biochem. J. 361 (Pt 1): 77–85. doi:10.1042/0264-6021:3610077. PMC 1222281. PMID 11742531.
- Ha Kim Y, Yeol Choi J, Jeong Y, Wolgemuth DJ, Rhee K (2002). "Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle". Biochem. Biophys. Res. Commun. 290 (2): 730–6. doi:10.1006/bbrc.2001.6212. PMID 11785960.
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- Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH (2003). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". J. Biol. Chem. 277 (51): 49408–16. doi:10.1074/jbc.M207069200. PMID 12386167.
- Faragher AJ, Fry AM (2004). "Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles". Mol. Biol. Cell. 14 (7): 2876–89. doi:10.1091/mbc.E03-02-0108. PMC 165683. PMID 12857871.
- Lou Y, Yao J, Zereshki A, Dou Z, Ahmed K, Wang H, Hu J, Wang Y, Yao X (2004). "NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling". J. Biol. Chem. 279 (19): 20049–57. doi:10.1074/jbc.M314205200. PMID 14978040.
- Noguchi K, Fukazawa H, Murakami Y, Uehara Y (2004). "Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells". J. Biol. Chem. 279 (31): 32716–27. doi:10.1074/jbc.M404104200. PMID 15161910.
- Fortna A, Kim Y, MacLaren E, Marshall K, Hahn G, Meltesen L, Brenton M, Hink R, Burgers S, Hernandez-Boussard T, Karimpour-Fard A, Glueck D, McGavran L, Berry R, Pollack J, Sikela JM (2006). "Lineage-specific gene duplication and loss in human and great ape evolution". PLOS Biol. 2 (7): E207. doi:10.1371/journal.pbio.0020207. PMC 449870. PMID 15252450.
- Lou Y, Xie W, Zhang DF, Yao JH, Luo ZF, Wang YZ, Shi YY, Yao XB (2004). "Nek2A specifies the centrosomal localization of Erk2". Biochem. Biophys. Res. Commun. 321 (2): 495–501. doi:10.1016/j.bbrc.2004.06.171. PMID 15358203.
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