RNAIII
RNAIIIRNAIII는 포도상구균 정족수 감지 농경 시스템 )의 P3 추진자가 필사한 안정적인 514 nt 규제 RNA이다. 농경기의 주요 이펙터로서, 엑소프로틴과 세포벽 관련 단백질을 인코딩하는 많은 S. aureus 유전자의 발현을 제어하며, 규제 단백질을 인코딩하는 RNAIII 대본은 26개의 아미노산 Δ-haemolyshin (Staphylocococcus delta독소) 펩타이드(Hld)도 인코딩한다.[5] RNAIIII는 많은 줄기 루프를 포함하고 있으며, 대부분은 조절된 유전자의 번역 시작에 관련된 샤인 달가노 수열과 일치한다.[5] 이러한 상호작용 중 일부는 억제성이고, 다른 것들은 자극성이며, 전자는 규제 단백질인 Rot이 있다.[6][7] 체외에서 RNAIII는 기하급수적으로 발현되어 표면 단백질, 특히 단백질 A의 번역을 억제하는 동시에 세포분해 효소나 시톨리신인 엑소프로틴의 번역을 자극한다.[8] 후자 중에는 중요한 맹독성 인자인 α-헬리신(또는 알파독소) (Hla)이 있는데, 이들의 번역 RNAIII는 hla mRNA 리더에 억제 폴드백 루프의 형성을 방지하여 활성화된다.[9]
참조
- ^ Novick RP, Ross HF, Projan SJ, Kornblum J, Kreiswirth B, Moghazeh S (October 1993). "Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule". The EMBO Journal. 12 (10): 3967–75. doi:10.1002/j.1460-2075.1993.tb06074.x. PMC 413679. PMID 7691599.
- ^ Waters LS, Storz G (February 2009). "Regulatory RNAs in bacteria". Cell. 136 (4): 615–28. doi:10.1016/j.cell.2009.01.043. PMC 3132550. PMID 19239884.
- ^ Janzon L, Arvidson S (May 1990). "The role of the delta-lysin gene (hld) in the regulation of virulence genes by the accessory gene regulator (agr) in Staphylococcus aureus". The EMBO Journal. 9 (5): 1391–9. doi:10.1002/j.1460-2075.1990.tb08254.x. PMC 551825. PMID 2328718.
- ^ Novick RP (June 2003). "Autoinduction and signal transduction in the regulation of staphylococcal virulence". Molecular Microbiology. 48 (6): 1429–49. doi:10.1046/j.1365-2958.2003.03526.x. PMID 12791129. S2CID 6847208.
- ^ a b Benito Y, Kolb FA, Romby P, Lina G, Etienne J, Vandenesch F (May 2000). "Probing the structure of RNAIII, the Staphylococcus aureus agr regulatory RNA, and identification of the RNA domain involved in repression of protein A expression". RNA. 6 (5): 668–79. doi:10.1017/S1355838200992550. PMC 1369947. PMID 10836788.
- ^ Geisinger E, Adhikari RP, Jin R, Ross HF, Novick RP (August 2006). "Inhibition of rot translation by RNAIII, a key feature of agr function". Molecular Microbiology. 61 (4): 1038–48. doi:10.1111/j.1365-2958.2006.05292.x. PMID 16879652. S2CID 229424.
- ^ Boisset S, Geissmann T, Huntzinger E, Fechter P, Bendridi N, Possedko M, et al. (June 2007). "Staphylococcus aureus RNAIII coordinately represses the synthesis of virulence factors and the transcription regulator Rot by an antisense mechanism". Genes & Development. 21 (11): 1353–66. doi:10.1101/gad.423507. PMC 1877748. PMID 17545468.
- ^ Dunman PM, Murphy E, Haney S, Palacios D, Tucker-Kellogg G, Wu S, et al. (December 2001). "Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci". Journal of Bacteriology. 183 (24): 7341–53. doi:10.1128/JB.183.24.7341-7353.2001. PMC 95583. PMID 11717293.
- ^ Morfeldt E, Taylor D, von Gabain A, Arvidson S (September 1995). "Activation of alpha-toxin translation in Staphylococcus aureus by the trans-encoded antisense RNA, RNAIII". The EMBO Journal. 14 (18): 4569–77. doi:10.1002/j.1460-2075.1995.tb00136.x. PMC 394549. PMID 7556100.
추가 읽기
- Chevalier C, Boisset S, Romilly C, Masquida B, Fechter P, Geissmann T, Vandenesch F, Romby P (March 2010). "Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation". PLOS Pathogens. 6 (3): e1000809. doi:10.1371/journal.ppat.1000809. PMC 2837412. PMID 20300607.
- Coelho LR, Souza RR, Ferreira FA, Guimarães MA, Ferreira-Carvalho BT, Figueiredo AM (November 2008). "agr RNAIII divergently regulates glucose-induced biofilm formation in clinical isolates of Staphylococcus aureus". Microbiology. Reading, England. 154 (Pt 11): 3480–3490. doi:10.1099/mic.0.2007/016014-0. PMID 18957601.
- Queck SY, Jameson-Lee M, Villaruz AE, Bach TH, Khan BA, Sturdevant DE, Ricklefs SM, Li M, Otto M (October 2008). "RNAIII-independent target gene control by the agr quorum-sensing system: insight into the evolution of virulence regulation in Staphylococcus aureus". Molecular Cell. 32 (1): 150–8. doi:10.1016/j.molcel.2008.08.005. PMC 2575650. PMID 18851841.
- Huntzinger E, Boisset S, Saveanu C, Benito Y, Geissmann T, Namane A, Lina G, Etienne J, Ehresmann B, Ehresmann C, Jacquier A, Vandenesch F, Romby P (February 2005). "Staphylococcus aureus RNAIII and the endoribonuclease III coordinately regulate spa gene expression". The EMBO Journal. 24 (4): 824–35. doi:10.1038/sj.emboj.7600572. PMC 549626. PMID 15678100.
- Chabelskaya S, Bordeau V, Felden B (April 2014). "Dual RNA regulatory control of a Staphylococcus aureus virulence factor". Nucleic Acids Research. 42 (8): 4847–58. doi:10.1093/nar/gku119. PMC 4005698. PMID 24510101.
